The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community and provides dedicated support to NCI’s intramural Center for Cancer Research (CCR). The dedicated units provide electron microscopy, protein characterization, protein expression, optical microscopy and genetics. These research efforts are an integral part of CCR at the Frederick National Laboratory for Cancer Research (FNLCR). CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.
The Sequencing Facility is a high throughput laboratory providing cutting edge next generation technologies to the National Cancer Institute.
- The candidate will be responsible for assisting with next generation (NGS) sequencing for the core facility. Current platforms include the Illumina HiSeq, NextSeq, MiSeq, PacBio and additional platforms in the future.
- All projects are of a highly specialized nature and require technical proficiency, problem solving skills and good judgment.
- Responsible for all ongoing daily activities including library prep and sequencer set up, maintenance of equipment, use of protocols, sample quality control and troubleshooting of problems with direct coordination of the supervisor.
- Requires adherence to strict safety protocols and may require the use of personal protective equipment.
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
- Possession of a Bachelor degree from an accredited college or university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
- In addition to educational requirements, a minimum of two (2) years progressively responsible job related biomedical experience.
- Must be able to obtain and maintain a security clearance
Candidates with these desired skills will be given preferential consideration:
- Four (4) years of experience in in related molecular biology experience. Experience with production of NGS libraries, sample and library quality control, and sequencing machine set up is essential.
- Proficiency utilizing onboard sequencer and commercial bioinformatics tools, core or service facility experience and the ability to assist in new protocol development. Experience with multiple next generation sequencing technologies including Illumina NextSeq or HiSeq is preferred.
- Working knowledge of DNA hybridization, ligation, fragmentation, endpolishing, PCR, automation using robotics, Agilent Bioanalyzer, nanodrop, qPCR, sterile/clean room techniques, and sample and process tracking through LIMS is also required.
- The ideal candidate must have the ability to organize and utilize reagents and samples for high-throughput analysis in support of genomic studies and two years proven experience in DNA sequencing, data review and stringent record keeping.
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