The In Vivo Evaluation Group, BTB, DTP is establishing both in vitro and in vivo tumor lines from patient biopsies and circulating tumor cells (CTCs) from the DCTD sponsored clinical trials with the goal of distributing well validated tumor tissue and in vitro cell cultures to researchers worldwide. Multiple in vitro cell culture samples consisting of mixed cells (fibroblast free heterogeneous tumor cells), clonally derived homogeneous tumor cells, organoids and fibroblast cell lines are being established from each patient derived sample.
The Research Technician will be responsible for:
- Aiding in the daily maintenance and cryopreservation of the various cultures grown in different medias
- Establishing organoid cultures from patient samples and from tumors isolated from mice
- Preparing all required medias, components and reagents (e.g., matrigel coated flasks) used by the laboratory
- Preparing cultures for the various validation assays (flow cytometry, cell sorting, Q RT PCR, soft agar clonal assays, RNA/DNA extraction, the STR identity assay, etc.)
- Characterizing the cultures for rates of proliferation and ability to grow as spheroids in serum free media
- Keeping the laboratory stocked
- Aiding in any other routine tasks as needed (cell photography, PowerPoint presentations, tube labeling, cell deliveries/pick-ups, etc.).
- The maintenance of proper records is critical as each patient derived cell mixture/clone/line will be passaged and expanded multiple times with the intent of delivering carefully documented samples to researchers worldwide.
All work will be conducted under strict BSL2 safety conditions.
Work is checked periodically for proper technique. Working hours are 6-9 am to 2:30-5:30 PM Monday – Friday, however, flexibility is required as patient samples arriving late must be processed and cells may require attention on weekends/holidays. Qualifications:
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
- Possession of a Bachelor's degree from an accredited college or university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
- No additional experience is required with a Bachelor’s degree
- Must be able to obtain and maintain a security clearance
Candidates with these desired skills will be given preferential consideration:
- Knowledge of sterile techniques
- Eukaryotic tissue culture skills
- Proficiency with Microsoft Windows and Microsoft Office
- Organizational skills and database management skills
- Ability to maintain precise records
- Ability to be flexible to meet changing needs
- Ability to work in a fast paced environment
- Good written and verbal communication skills
Leidos is a global science and technology solutions leader working to solve the world’s toughest challenges in the defense, intelligence, homeland security, civil, and health markets. The company’s 33,000 employees support vital missions for government and commercial customers. Headquartered in Reston, Virginia, Leidos reported pro forma annual revenues of approximately $10 billion for the fiscal year ended January 1, 2016 after giving effect to the recently completed combination of Leidos with Lockheed Martin's Information Systems Global Solutions business (IS GS). For more information, visit www.Leidos.com. The company’s diverse employees support vital missions for government and commercial customers. Qualified women, minorities, individuals with disabilities and protected veterans are encouraged to apply. Leidos will consider qualified applicants with criminal histories for employment in accordance with relevant Laws. Leidos is an Equal Opportunity Employer.